Short interfering RNA-mediated inhibition of herpes simplex virus type 1 gene expression and function during infection of human keratinocytes |
| |
Authors: | Bhuyan Prakash K Karikò Katalin Capodici John Lubinski John Hook Lauren M Friedman Harvey M Weissman Drew |
| |
Affiliation: | Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA. bhuyan@prodigy.net |
| |
Abstract: | RNA interference (RNAi) is an antiviral mechanism that is activated when double-stranded RNA is cleaved into fragments, called short interfering RNA (siRNA), that prime an inducible gene silencing enzyme complex. We applied RNAi against a herpes simplex virus type 1 (HSV-1) gene, glycoprotein E, which mediates cell-to-cell spread and immune evasion. In an in vitro model of infection, human keratinocytes were transfected with siRNA specific for glycoprotein E and then infected with wild-type HSV-1. RNAi-mediated gene silencing reproduced the small plaque phenotype of a gE-deletion mutant virus. The specificity of gene targeting was demonstrated by flow cytometry and Northern blot analyses. Exogenous siRNA can suppress HSV-1 glycoprotein E expression and function during active infection in vitro through RNAi. This work establishes RNAi as a genetic tool for the study of HSV and provides a foundation for development of RNAi as a novel antiviral therapy. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|