The primary structure of an endocuticular protein from two locust species,Locusta migratoria and Schistocerca gregaria,determined by a combination of mass spectrometry and automatic Edman degradation |
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| Affiliation: | 1. Department of Molecular Biology, 55 Campusvej, DK-5230 Odense M, Denmark;2. August Krogh Institute, 13 Universitetsparken, DK-2100 Copenhagen Ø, Denmark;1. Animal Genetics, University of Tübingen, Germany;2. Biozentrum, University of Würzburg, Germany;3. Max-Planck Institute for Developmental Biology, Tübingen, Germany;4. iBV, Université Nice Sophia-Antipolis, France;1. Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark;2. Danish Medicines Agency, Axel Heides Gade 1, 2300 Copenhagen S, Denmark;3. Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark;1. Institute of General Zoology and Endocrinology, Ulm University, Helmholtzstrasse 8/1, 89081, Ulm, Germany;2. Helmholtz Centre for Infection Disease, Cellular Proteome Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany;3. Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University Basel, Mattenstrasse 26, 4058, Basel, Switzerland;1. Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Denmark;2. Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark;3. Department of Biomedical Sciences, University of Prince Edward Island, Charlottetown PEI, Canada;4. Pharmaceutical Research Center of Excellence, School of Pharmacy (Pharmacology), North-West University, Potchefstroom, South Africa;5. Department of Physiology at Institute of Neuroscience and Physiology, University of Gothenburg, Sweden;6. Comparative Medicine Lab, Department of Clinical Medicine, Health, Aarhus University, Denmark;7. Section for Zoophysiology, Department of Bioscience, Aarhus University, Denmark;8. Department of Forensic Medicine, Health, Aarhus University, Denmark;9. Department of Orthopaedic Surgery & Traumatology, Odense University Hospital, Denmark;10. Department of Clinical Research, University of Southern Denmark;1. College of Information and Electrical Engineering, China Agricultural University, Beijing, PR China;2. University of Ohio State, Columbus, USA;3. Bio-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China;1. Laboratory of Biology, Sapporo Campus, Hokkaido University of Education, Sapporo, 002-8502, Japan;2. Research Center for Structural Materials, National Institute for Materials Science, Tsukuba, 305-0044, Japan |
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| Abstract: | The complete primary structures of two variants of a protein, Abd-5, isolated from the endocuticles of the migratory locust Locusta migratoria and the desert locust Schistocerca gregaria, have been determined. The proteins from the two species are N-terminally blocked with pyroglutamic acid. Their sequences differed only in two positions. Comparison of the sequences to those of other cuticular proteins shows that moderate homologies exist to 11 other cuticular proteins from insects representing four different orders. Amino acid residues in certain positions appear to be strictly conserved. |
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