Construction and preliminary analysis of the ICRF human P1 library |
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| Affiliation: | 1. Institute of Botany, Czech Academy of Science, Průhonice, Czech Republic;2. Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic;3. Global Change Research Institute CAS, Brno, Czech Republic;4. Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic;5. Czech Hydrometeorological Institute, Prague, Czech Republic;6. Czech Geological Survey, Klárov 3, 118 21 Prague, Czech Republic;7. IFER – Institute of Forest Ecosystem Research, Jílové u Prahy, Czech Republic;8. Charles University, Faculty of Science, Department of Physical Geography and Geoecology, Albertov 6, 12843 Prague, Czech Republic |
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| Abstract: | P1 clone libraries have now been established as effective complements to cosmid and yeast artificial chromosome libraries in long-range mapping projects. To allow general access to P1 clones, we have constructed human and mouse P1 libraries. Clones have been picked into microtiter plates and used to prepare high-density filter grids, providing an efficient and easy screening system. Filters are being made available to other laboratories through the Reference Library System. In this work, we have developed a reliable protocol for generating P1 clones, based on the use of pulsed-field gel electrophoresis for size selection of DNA. A 1.2× genome coverage human library has been produced using this method. A preliminary analysis of this library is described. |
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