Computer-aided on-line monitoring of physiological variables in suspended cell cultures of Perilla frutescens in a bioreactor |
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| Institution: | 1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, Hungary;2. Biotechnology Development Department, Gedeon Richter Plc., Gyömrői út 19-21, H-1103, Budapest, Hungary;1. PhD, Advanced Technology and Research, Arup, San Francisco, CA, USA;2. PhD, T.Y. Lin International, San Francisco, CA, USA;1. National Institute for Public Health and the Environment (RIVM), PO Box 1, 3720 BA Bilthoven, The Netherlands;2. Delft University of Technology, P.O. Box 5, 2600 AA Delft, The Netherlands;3. Utrecht University, P.O. Box 80125, 3508 TC Utrecht, The Netherlands;4. Institute for Risk Assessment Sciences, P.O. Box 80178, 3508 TD Utrecht, The Netherlands;5. Waternet, P.O. Box 94370, 1090 GJ Amsterdam, The Netherlands |
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| Abstract: | A computer-aided on-line real-time monitoring system for plant cell bioprocesses was established and applied to the cultivation of Perilla frutescens plant cells in a bioreactor. This system calculated several informative process variables which were useful for the identification of the physiological states of the plant cells during cultivation. Some variables, such as the respiratory quotient (RQ), pH, and specific carbon dioxide evolution rate (SCER), could be used for the identification of the growing phase of cell cultures. The results also suggest that the oxygen uptake rate (OUR) and the specific OUR (SOUR) may depend on the accumulation of anthocyanin (a secondary metabolite) in P. frutescens cell cultures. |
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