Continuous production of an antibiotic polypeptide (nisin) by Lactococcus lactis using a bioreactor coupled to a microfiltration module |
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| Institution: | 1. Institute of Nutrition and Functional Foods (INAF) and Department of Food Sciences, Université Laval, Québec, QC, Canada;2. Laboratory of Food Processing and ElectroMembrane Processes (LTAPEM), Université Laval, Québec, QC, Canada;3. Département de Mathématiques et de Statistique, Faculté des Sciences et de Génie, Université Laval, Québec, QC, Canada;1. Department of Bioresource Sciences, Andong National University, Andong 36729, Republic of Korea;2. Bongwha County, 1111 Bonghwa-ro, Bonghwa 36239, Republic of Korea;3. Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA, Wanju 55365, Republic of Korea;4. Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Republic of Korea;1. Department of General Surgery, University of Michigan, Ann Arbor, Michigan;2. Department of Surgery, Beaumont Hospital Dearborn, Dearborn, Michigan;3. Department of Surgery, Henry Ford Health System, Detroit, Michigan;1. School of Ecology and Environment, School of Water Conservancy Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China;2. College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, PR China;3. School of Pharmaceutical Sciences, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, Henan Province, 450001, China;1. Biodeterioration Control Associates, Inc, P.O. Box 3659 Princeton, New Jersey, NJ, 08543-3659, USA;2. Conidia Bioscience Ltd, Bakeham Lane, Egham, Surrey, TW20 9TY, UK;3. LuminUltra Technologies, Ltd, 520 King Street, Fredericton, NB E3B 6G3, Canada |
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| Abstract: | The continuous production of nisin, an antibiotic polypeptide, by Lactococcus lactis in a bioreactor system coupled to a microfiltration module is described. Nisin productivity with respect to both cultivation time (ND) and the quantity of glucose consumed (ND/Sf) in continuous production was enhanced by maintaining a low concentration of lactic acid in the broth. A maximum ND of 7.80 × 104 U·l?1·h?1 and ND/Sf of 5.20 × 103 U·g?1·h?1 were obtained when the glucose concentration in the feed medium was 15 g/l. These values represent about 4.1- and 4.5-fold increases, respectively, over those obtained in batch culture. |
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