Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum) |
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| Institution: | 1. Chair of Computer Architecture and Parallel Systems, Department of Informatics, Technical University of Munich, Germany;2. Department of Computer Science / Mathematics, University of Exeter, EX4 4QF, United Kingdom;3. Univ. Grenoble Alpes, CNRS, ISTerre, Grenoble F-38000, France;4. CISL/NCAR, Boulder, USA;1. Department of Food Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;2. BioCentre Ltd., Borongajska street 83h, Zagreb, Croatia;3. Institute of Physics Zagreb, Bijenička street 46, 10000 Zagreb, Croatia |
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| Abstract: | Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum, Kinosternidae) were analyzed by gas chromatography-mass spectrometry (GC-MS) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Analysis by GC-MS indicates 2,3-dihydroxypropanal and C3–C24 free or esterified fatty acids. Analysis by SDS-PAGE indicates a major protein component with an approximate molecular mass of 60 kDa and minor components ranging from ca. 23 to 34 kDa. The major component of K. subrubrum glandular secretions exhibits a mobility that matches that of the Kemp's ridley sea turtle (Lepidochelys kempi, Cheloniidae), suggesting that these proteins are evolutionarily conserved. |
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