Continuous production of human erythropoietin by immobilized recombinant L-929 cells期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Continuous production of human erythropoietin by immobilized recombinant L-929 cells

Affiliation:	1. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Luis Encinas y Rosales, Hermosillo, Sonora 83000, Mexico;2. Dipartimento di Scienze Farmaceutiche, Università degli Studi di Perugia—Via del Liceo, 1, 06123 Perugia, Italy;1. Department of Gynecology and Obstetrics, General Hospital of Ciudad Victoria “Dr. Norberto Treviño Zapata”, Ciudad Victoria, Tamaulipas, Mexico;2. Department of Anesthesiology, General Hospital of Ciudad Victoria “Dr. Norberto Treviño Zapata”, Ciudad Victoria, Tamaulipas, Mexico;3. Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico;1. Laboratory of Integrative Genomics, Department of Integrative Biology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India;2. Faculty of Allied Health Sciences, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, India;3. Department of Biomedical Sciences, College of Health and Sciences, QU Health, Qatar University, Doha, Qatar;1. Universidad Autónoma del Estado de México, Facultad de Química, Programa de Maestría en Ciencias Químicas, Paseo Colón esq. Paseo Tollocan s/n, Toluca, México CP 50120, Mexico;2. Universidad Autónoma del Estado de México, Facultad de Química. Laboratorio de Investigación y Desarrollo de Materiales Avanzados (LIDMA), Carretera Toluca-Atlacomulco Km 14.5, Unidad San Cayetano, Toluca, México CP 50100, Mexico;3. Universidad Autónoma del Estado de México, Facultad de Química, Centro Conjunto de Investigación en Química Sustentable UAEM-UNAM, Carretera Toluca-Atlacomulco Km 14.5, Unidad San Cayetano, Toluca, México CP 50100, Mexico;4. Departamento de Física, Instituto Nacional de Investigaciones Nucleares, Carretera México‑Toluca S/N, La, Marquesa, CP 52750 Ocoyoacac, Estado de México, Mexico

Abstract:	Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 10⁷ cells/cm³-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

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