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Purification and characterization of a poly-l-lysine-activated serine endoprotease from Lumbricus rubellus
Affiliation:1. Department of Molecular Biology and SRC for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea;2. Department of Biochemistry, College of Medicine, Inha University, Inchon 402-751, Korea;1. Oil, Gas, and Biofuels Research Group (PGBio), Postgraduate Program of Chemical Engineering (PPEQ), Federal University of Bahia (UFBA), R. Prof. Aristides Novis, 2, 2° floor, Federação, CEP 40210-630, Salvador, BA, Brazil;2. Institute and Center for Energy and Environment (CIENAM), Federal University of Bahia (UFBA), R. Av. Adhemar de Barros, s/n, 2° floor, Ondina, CEP 40301-110, Salvador, BA, Brazil;3. Engineering School, Salvador University (UNIFACS), Av. Tancredo Neves, 2131, Caminho das Árvores, CEP 40231-902, Salvador, BA, Brazil;4. Postgraduate Program of Geochemistry, Federal University of Bahia (UFBA), R. Av. Adhemar de Barros, s/n, 2° floor, Ondina, CEP 40301-110, Salvador, BA, Brazil;1. Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Analytical Chemistry, TR-03100, Afyonkarahisar, Turkey;2. Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Pharmacognosy, TR-03100, Afyonkarahisar, Turkey;3. Cukurova University, Faculty of Science and Literature, Department of Biology, TR-01330, Adana, Turkey;4. Kilis 7 Aralik University, Faculty of Science and Literature, Department of Molecular Biology and Genetics, TR-79000, Kilis, Turkey;1. University of Chemistry and Technology Prague, Prague, Czech Republic;2. Institute on Membrane Technology, ITM-CNR, Rende, Italy;3. Nanoscience Institute of Aragon (INA), University of Zaragoza, Zaragoza, Spain;1. Department of Psychiatry and Human Behavior, Alpert Medical School of Brown University, Providence, Rhode Island;2. Centers of Preventive Medicine, The Miriam Hospital, Providence, Rhode Island;3. Butler Hospital, Providence, Rhode Island;4. Department of Human Development & Family Sciences, Rudd Center for Food Policy & Obesity, University of Connecticut, Storrs, Connecticut;5. Department of Psychological Sciences, Institute for Collaboration on Health, Intervention, & Policy, University of Connecticut, Storrs, Connecticut;6. Surgical Weight Loss Center, Hartford Healthcare, Hartford, Connecticut
Abstract:An endoprotease in earthworm (Lumbricus rubellus) is purified to apparent homogeneity using 125I-lactalbumin as a substrate. The protease has a molecular mass of 27 kDa and is markedly activated by poly-l-lysine or poly-l-arginine. It is a chymotrypsin-like serine protease. Its activity is distributed to coelomic fluid but relatively little to coelomocytes.
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