In vivo effects of the anesthetic,benzocaine, on liver microsomal cytochrome P450 and mixed-function oxidase activities of gilthead seabream (Sparus aurata) |
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| Affiliation: | 1. Department of Rheumatology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China;2. Department of Rheumatology, The First Affiliated Hospital of University of South China, HengYang 421001, PR China;1. Subdirection of Research and Technology, Agro-Technological Institute of Castilla y León, Consejería de Agricultura y Ganadería, Finca de Zamadueñas, Ctra. Burgos km. 119, Valladolid 47071, Spain;2. Research Group of Aquaculture and Biodiversity, Institute of Animal Science and Technology, Universitat Politècnica de València, Camino de Vera, 14, Valencia 46071, Spain;3. Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell''Università 16 Agripolis, Legnaro 35020, Italy |
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| Abstract: | Gilthead seabreams were exposed to benzocaine, 4-aminobenzoic acid ethyl ester, 57 mg/l in sea water for 3 min, daily, for 2 or 3 consecutive days. The fish were killed 20 hr after the last treatment. Benzocaine treatment for 2 or 3 days resulted in 57% and 67% inhibition of liver microsomal aniline 4-hydroxylase and ethylmorphine N-demethylase activities,respectively. The total cytochrome P450 content of fish liver microsomes was unaltered following the 2-day benzocaine treatment. However, additional 3 min benzocaine treatment on day 3 reduced cytochrome P450 level by 50%. Benzocaine produced type II difference spectra with rabbit liver microsomes. Difference spectra of fish liver microsomes elicited by benzocaine were complex. The position of peak and intensity were greatly influenced by the concentration of benzocaine. |
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