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Stimulation of the rate of l-lactate fermentation using Lactococcus lactis IO-1 by periodic electrodialysis
Institution:1. Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, Mexico;2. Nanoscience & Nanotechnology Program, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, Mexico;1. Graduate School of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan;2. Sanbongi High School of Agriculture, Towada, Aomori 034-8578, Japan;1. Department of Polymer Science and Rubber Technology, Cochin University of Science and Technology, Cochin, Kerala, 682022 India;2. Department of Chemistry, Maharajas College, Ernakulam, Kerala, 682011, India;3. Department of Chemistry, Sree Kerala Varma College, Thrissur, Kerala, 680011, India;1. Centenary Institute, The University of Sydney, Australia;2. The University of Sydney, Discipline of Pathology Faculty of Medicine and Health, Australia;3. The University of Sydney, Central Clinical School Faculty of Medicine and Health and Marie Bashir Institute, Australia;4. The University of Sydney, School of Chemistry, Australia
Abstract:Lactic acid fermentation in glucose medium with periodic electrodialysis by Lactococcus lactis IO-1 was examined. The fermentation time was reduced considerably, compared with the time required for ordinary built-in electrodialysis fermentation with a microfilter module (ED-MF). Fermentation with an initial glucose concentration of 80 g/l was completed within 18 h, about 50% of the time required with an ED-MF. The maximum productivity of this novel system was about two-fold that of the ordinary ED-MF system even when the lactate concentration in broth was higher than in the ED-MF. The H2 gas produced from the ED-MF made the culture redox potential (CRP) lower than in the novel system. Online culture redox potential was monitored and higher CRP indicated a higher fermentation rate.
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