A new biosynthetic pathway of porphyrins from isopropanol |
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| Affiliation: | 1. Department of Medicinal Chemistry, Meiji College of Pharmacy, 1-22-1 Yato-cho, Tanashi-shi, Tokyo 188 Japan;2. Central Technical Research Laboratory, Nippon Oil Company, Ltd., 8, Chidori-cho, Naka-ku, Yokohama 231, Japan;1. School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, 430205, China;2. School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, 430205, China;1. Department of Chemical and Biochemical Engineering and the Key Lab for Synthetic Biotechnology of Xiamen City, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;2. University of Rwanda-College of Education, P.O. Box 5039, Kigali, Rwanda;3. Rwanda Education Board, P.O. Box 3817, Kigali, Rwanda;4. College of Energy, Xiamen University, Xiamen 361005, China;1. Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, CEP – 05508-000 São Paulo, SP, Brazil;2. Laboratório de Processos Metalúrgicos, Centro de Tecnologia em Metalurgia e Materiais, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, CEP – 05508-901 São Paulo, SP, Brazil |
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| Abstract: | A new biosynthetic pathway, which can produce both vitamin B12 and large amounts of porphyrins from isopropanol, was identified in Arthrobacter hyalinus using carbon-13 stable isotope tracer techniques and carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy. Studies on the incorporation of [2-13C]isopropanol, [1- or 2-13C]sodium acetate, l-[1-13C]glutamate, and [1-, 2-, 3-, 4-, 5-13C]5-aminolevulinic acid into uroporphyrinogen III showed that isopropanol was metabolized into uroporphyrinogen III through acetyl CoA and that 5-aminolevulinic acid was produced from l-glutamic acid and not via Shemin's pathway. |
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