Screening cosmid libraries with oligonucleotides corresponding to splice-site consensus sequences |
| |
| Institution: | 1. Carbon Nanotechnology Lab, Department of Physics, National Institute of Technology, Durgapur 713209, WB, India;2. Department of Physics, SRM University, Andhra Pradesh 522502, India;3. Centre of Excellence in Advanced Materials, National Institute of Technology, Durgapur 713209, WB, India;4. Department of Chemistry, SRM University, Andhra Pradesh 522502, India;5. Organic Electronics Research Center, Ming-Chi University of Technology, 84 Gungjuan Rd., New Taipei City 24301, Taiwan;6. Department of Electronic Engineering, Ming-Chi University of Technology, New Taipei City 24301, Taiwan;7. Center for Nanofibers and Nanotechnology, National University of Singapore, Singapore 117576, Singapore;8. School of Electrical and Electronic Engineering, Nanyang Technological University, Block S2, Nanyang Avenue, Singapore 639798, Singapore;9. Sunkonnect, 1 Cleantech Loop, Singapore 637141, Singapore;1. Department of Soil Science, Faculty of Agricultural Engineering and Technology, University College of Agriculture and Natural Resources, University of Tehran, 31587-77871 Iran;2. Horticulture Major, Division of Applies Life Science (BK21 Plus Program), Graduate School, Gyeongsang National University, Jinju 52828, Republic of Korea;1. Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Sudan;2. Botany Department, Faculty of Science, University of Khartoum, Sudan;1. Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China;2. National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop Improvement, College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China;3. Department of Soil and Environmental Sciences, University College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan;4. CAS-Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China;5. Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;6. Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434023, China;1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China;3. Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil Science, University of Göttingen, Büsgenweg 2, Göttingen, 37077, Germany;4. Agro-Technology Institute, RUDN University, Moscow, Russia;5. Institute of Environmental Sciences, Kazan Federal University, 420049, Kazan, Russia |
| |
| Abstract: | To facilitate the identification of genes within genomic DNA, we have developed a method based on the use of short oligonucleotides designed from the consensus sequences of splice sites. We describe here the hybridization and washing conditions under which such oligonucleotides can be used to screen cosmid libraries. We confirm the presence of genes within cosmids identified by screening with one oligonucleotide by showing that DNA isolated from such cosmids will hybridize to another splice-site oligonucleotide. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
