A novel tyrosine kinase activity in the cotton leafworm,Spodoptera littoralis |
| |
| Affiliation: | 1. Environment Department, University of York, York YO10 5DD, UK;2. Fundación Talking Oceans, Carrera 16 #127-81, Bogotá, Colombia;3. Smithsonian Tropical Research Institute, Balboa, Ancón, Panamá City, Panama;1. Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstr. 14, 35390 Giessen, Germany;2. Faculty of Biology and Chemistry, Justus Liebig University, Ludwigstr. 23, 35390 Giessen, Germany;3. Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Project Group Bioresources, Winchester Str. 2, 35394 Giessen, Germany;4. Department of Chemical Engineering, Kansas State University, 1005 Durland Hall, Manhattan, KS 66506, USA;1. School of Architecture and Urban Planning, Shenzhen University, China;2. Department of Architecture, National University of Singapore, Singapore;3. Department of Real Estate, National University of Singapore, Singapore;1. Department of Medicine III, University Hospital, LMU Munich, Munich, Germany;2. Cancer- and Immunometabolism Research Group, Gene Center, LMU Munich, Munich, Germany;3. Evidence-based Medicine, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf, Faculty of Medicine and University Hospital Cologne, University of Cologne, Germany;4. Comprehensive Cancer Center Munich (CCCM), Munich, Germany;5. German Cancer Consortium (DKTK), Partner Site Munich, and German Cancer Research Center (DKFZ), Heidelberg, Germany;1. Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain;2. Institute of Sustainable Processes, Dr. Mergelina s/n, 47011 Valladolid, Spain;3. Department of Hydraulic and Environmental Engineering, Federal University of Ceará, Fortaleza, Ceará, Brazil |
| |
| Abstract: | A rapid, highly-specific ELISA for tyrosine kinases readily detects such activity in crude extracts prepared from rat mammary epithelial and fibroblastic cells that have been stimulated with epidermal growth factor or basic fibroblast growth factor. Tyrosine kinase activity is also found in extracts of pupae of the cotton leafworm, Spodoptera littoralis. Both the mammalian and the insect tyrosine kinases are ATP-dependent. Both cytosol and membrane-associated (Triton-X-100-soluble) fractions of Spodoptera littoralis pupae contain tyrosine kinase activity. The growth factor-stimulated tyrosine kinase activities in extracts of growth factor-stimulated rat mammary cells are inhibited by genistein and an analogue of erbstatin: methyl 2,5-dihydroxycinnamate. However, the tyrosine kinase activities present in pupae of Spodoptera littoralis are not sensitive to these inhibitors, suggesting that the major tyrosine kinases of Spodoptera littoralis pupae may be distinct from the growth factor-stimulated mammalian tyrosine kinases. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
