柞蚕Septin基因的克隆及表达分析

Septin蛋白参与了细胞分裂、细胞内物质转运、细胞周期调控及细胞凋亡等生理反应,并且发现与肿瘤发生、神经功能障碍和病原物侵染的过程直接相关。研究克隆了柞蚕septin基因全长,序列分析表明septin基因全长1904 bp,开放阅读框长度为1137 bp,编码378个氨基酸。多重序列比对分析表明柞蚕septin与家蚕及黑脉金斑蝶septin相似性最高,聚为一类。实时定量PCR结果表明柞蚕septin基因在各个组织中都有表达,但在血细胞中表达量最高,其次是表皮,而在丝腺中表达量最低。通过构建原核表达载体,柞蚕septin蛋白在大肠杆菌中成功诱导表达。

Cloning and expression analysis of a septin gene from Antheraea pernyi

Septin gene family participates in cytokinesis,vesicle trafficking,cell cycle regulation and apoptosis procession. And septins were reported also involving in the pathogenesis of different diseases including neoplasia,neurodegeneration and pathogen infections. A septin gene from A. pernyi was cloned. The c DNA of septin in A. pernyi was 1904 bp in length,with 1137 bp ORF encoding a protein of 378 amino acids. The sequence alignment analysis indicated that the deduced amino sequence of septin in A. pernyi shared high identity with septin in Bombyx mori and Danaus plexippus. Real time PCR results showed that septin gene expressed in all tested tissues of A. pernyi,but highest in hemocytes and lowest in silk gland. By construction prokaryotic expression vector,septin protein was successful expressed in Escherichia coli induced by IPTG.