Phytase production by the yeast, Pichia anomala |
| |
Authors: | Ashima Vohra T. Satyanarayana |
| |
Affiliation: | (1) Department of Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110 021, India |
| |
Abstract: | Pichia anomala, isolated from dried flower buds of Woodfordia fruticosa, produced a high activity of an intracellular phytase, at 68 U per g dry biomass, when grown at 20 °C for 24 h in a medium containing glucose (40 g l–1) and beef extract (10 g l–1) supplemented with Fe2+ (0.15 mM). Partially purified phytase was optimally active at 60 °C and pH 4 with a half life of 7 days at 60 °C. It retained 85% of its activity at 80 °C for 15 min. The enzyme is suitable for supplementing animal feeds to improve the availability of phosphate from phytate. |
| |
Keywords: | phytase Pichia anomala yeast |
本文献已被 SpringerLink 等数据库收录! |
|