Minocycline attenuates neuronal apoptosis and improves motor function after traumatic brain injury in rats |
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Authors: | Jihong He Jian Mao Lei Hou Shimin Jin Xiaodong Wang Zhaoqi Ding Zhene Jin Hua Guo Rongxiao Dai |
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Institution: | 1)Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No. 1 Chengbei Road, Jiading District, Shanghai 200127, P.R. China;2)Ningbo Hangzhou Bay Hospital, 1155 Binhai 2nd Road, Cixi, Ningbo 315336, P.R. China;3)The Second Affiliated Hospital of Nanchang University, No. 1 Minde Road, Donghu District, Nanchang 330006, P.R. China |
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Abstract: | Minocycline is a type of tetracycline antibiotic with broad-spectrum antibacterial activity that has been demonstrated to protect the brain against a series of central nervous system diseases. However, the precise mechanisms of these neuroprotective actions remain unknown. In the present study, we found that minocycline treatment significantly reduced HT22 cell apoptosis in a mechanical cell injury model. In addition, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining confirmed the neuroprotective effects of minocycline in vivo through the inhibition of apoptosis in a rat model of controlled cortical impact (CCI) brain injury. The western blotting analysis revealed that minocycline treatment significantly downregulated the pro-apoptotic proteins BAX and cleaved caspase-3 and upregulated the anti-apoptotic protein BCL-2. Furthermore, the beam-walking test showed that the administration of minocycline ameliorated traumatic brain injury (TBI)-induced deficits in motor function. Taken together, these findings suggested that minocycline attenuated neuronal apoptosis and improved motor function following TBI. |
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Keywords: | apoptosis minocycline motor function traumatic brain injury (TBI) |
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