D-malic acid production from DL-malic acid by enantiospecific assimilation with Acinetobacter lwofii |
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| Authors: | Masayo Miyama Kiyoshi Nakayama |
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| Affiliation: | (1) Zama Research Laboratories, Bior Inc., Zama, 228, 5-5410 Kanagawa, Japan |
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| Abstract: | Summary Acinetobacter lwofii ATCC 9036 assimilated L-malic acid eantiospecifically and left D-malic acid when grown in a medium containing DL-malic acid. The optical purity of the D-malic acid isolated from the culture filtrate was 100%. When the organism was incubated at 26°C, 220 r.p.m. in a Erlenmeyer flask containing 100g/l of disodium maleate, L-malic acid was completely consumed during 7 days incubation and D-malic acid remained at the concentration of 35g/l. |
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