Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum |
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Authors: | Min-Kyoung Kang Jin-Hee Eom Yunje Kim Youngsoon Um Han Min Woo |
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Institution: | 1. Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5 Seongbuk-gu, Seoul, 136-791, Republic of Korea 2. Department of Chemistry, Korea University, 145 Anam-ro Seongbuk-gu, Seoul, 136-701, Republic of Korea 4. Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro Yuseong-gu, Daejeon, 305-350, Republic of Korea 3. Green School, Korea University, 145 Anam-ro Seongbuk-gu, Seoul, 136-701, Republic of Korea
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Abstract: | Pinene is a monoterpenes (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g?1 cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production. |
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