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Characterization of 28 microsatellite loci in the European roller Coracias garrulus (Coracidae,AVES) |
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| Authors: | David Martín-Gálvez Mercedes Molina-Morales Deborah A Dawson Deseada Parejo Juan Gabriel Martínez Jesús M Avilés |
| Institution: | 1. Department of Funcional and Evolutionary Biology, Estación Experimental de Zonas áridas (CSIC), Almería, 04120, Spain 2. Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK 4. European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus Hinxton, Cambridge, CB10 1SD, UK 3. Departamento de Biología Animal, Universidad de Granada, Granada, 18071, Spain |
| Abstract: | One hundred and forty-four existing microsatellite avian loci were tested for polymorphism in two to four unrelated European rollers. This allowed the identification of 28 unique polymorphic loci that we characterized in 34 unrelated individuals from a population in Guadix, Spain. The putative chromosomal locations of all the polymorphic loci in European rollers could be assigned based on the location of their orthologues in the assembled zebra finch and chicken genomes. All loci were assigned to autosomes based on predictive mapping and/or the presence of female heterozygotes, except CAM-11 that was assigned to the Z chromosome. By using a selected subset of 15 autosomal loci, the combined non-exclusion probability for identity was 4.7E-09. |
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