| G-蛋白 Rab3a 对神经生长抑制因子 (GIF) 抑制神经元细胞生长作用的影响 |
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| 引用本文： | 庞立岩,茹炳根. G-蛋白 Rab3a 对神经生长抑制因子 (GIF) 抑制神经元细胞生长作用的影响[J]. 生物化学与生物物理进展, 2005, 32(11): 1069-1074 |
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| 作者姓名： | 庞立岩 茹炳根 |
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| 作者单位： | 北京大学生命科学学院,蛋白质工程与植物基因工程国家重点实验室,北京,100871 |
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| 基金项目： | 国家自然科学基金资助项目(30450009). |
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| 摘 要： | 为了深入研究 Rab3a 和神经生长抑制因子 (GIF) 的相互作用，在大鼠海马神经元细胞原代培养体系中，用 MTT 还原法定量研究了 Rab3a 对 GIF 神经生长活性的影响，发现 Rab3a 可以替代脑提取物而使 GIF 发挥神经生长抑制活性 . 接着又利用多克隆抗体方法研究了 GIF 与 Rab3a 的相互作用，结果说明 Rab3a 是 GIF 发挥神经生长抑制活性所必需的蛋白质，并且它们的相互作用受空间位置的因素影响较大 . 在随后对它们相互作用的机理和可能的生物学意义进行了讨论 .
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| 关 键 词： | Rab3a ， GIF ，相互作用，大鼠海马神经元细胞培养， MTT 还原测定法 |
| 收稿时间： | 2005-05-17 |
| 修稿时间： | 2005-05-172005-09-28 |
The Influence of G-Protein Rab3a on The Activity of The Neuronal Inhibitory Factor(GIF) |
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| PANG Li-Yan and RU Bing-Gen. The Influence of G-Protein Rab3a on The Activity of The Neuronal Inhibitory Factor(GIF)[J]. Progress In Biochemistry and Biophysics, 2005, 32(11): 1069-1074 |
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| Authors: | PANG Li-Yan and RU Bing-Gen |
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| Affiliation: | The National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China;The National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China |
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| Abstract: | MTT reduction assay was used for the quantitative detection of the inhibitory activity of GIF in the rat hippocampal nerve call culture system. Evaluation of the influence of presence of Rab3a on the inhibitory activity of GIF indicated that Rab3a is enough to support the inhibitory activity of GIF instead of AD brain extract. Subsequently, the influence of antibody of GIF or Rab3a on the interaction between GIF and Rab3a was examined. The results indicated that Rab3a was most important to the inhibitory activity of GIF, and the interaction between GIF and Rab3a deeply depend on the spatial conditions. The mechanism and significance of the interaction between GIF and Rab3a are discussed. |
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| Keywords: | Rab3a neuronal growth inhibitory factor (GIF) interaction rat hippocampal nerve cell culture system MTT reduction assay |
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