Human eosinophils express CR1 and CR3 complement receptors for cleavage fragments of C3 |
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| Authors: | E Fischer M Capron L Prin J P Kusnierz M D Kazatchkine |
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| Affiliation: | 1. Division of Animal Genetics and Breeding, ICAR- IVRI, Izatnagar-243122, Bareilly, U.P., India;2. ICAR-National Research Centre on Pig, Rani-781131, Guwahati, Assam, India;3. Animal Health Division, ICAR- CIRG, Makhdoom-281112, U.P., India;1. Disease Glycomics Team, Global Research Cluster, RIKEN, Saitama 351-0198, Japan;2. Central Research Laboratories, Seikagaku Corporation, Tokyo 207-0021, Japan;3. Center for Highly Advanced Integration of Nano and Life Sciences (G-CHAIN), Gifu University, Gifu 501-1193, Japan;4. Structural Glycobiology Team, Global Research Cluster, RIKEN, Saitama 351-0198, Japan;5. Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan;6. University of Veterinary Medicine Hannover, Research Center for Emerging Infections and Zoonoses (RIZ), Infection Immunology, Hannover 30559, Germany;7. Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam 14424, Germany;8. Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin, Berlin 14195, Germany;9. Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan;10. Division of Pulmonary Medicine, Department of Medicine, Keio University, School of Medicine, Tokyo 160-8582, Japan;11. The Respiratory Care Clinic, Nippon Medical School, Tokyo 102-0074, Japan;12. Laboratory of Molecular Immunology, Immunology Frontier Research Center, Osaka University, Suita 565-0871, Japan;13. Division of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan |
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| Abstract: | The functional and antigenic characteristics of C3 receptors expressed on human eosinophils were investigated using rosette assays with sheep erythrocytes coated with C3 fragments and flow cytometric analysis of cells stained with anti-receptor antibodies. Purified peripheral blood eosinophils from 13 patients with hypereosinophilia expressed CR1 antigens. In 8 patients, a mean of 14 + 9.5% eosinophils formed C3b-dependent rosettes that were inhibited by F(ab')2 anti-CR1 antibodies. This number increased to 33% following stimulation with leukotriene B4 (LTB4) (10(-7) M). Similar numbers of C3b rosettes were formed by hypodense and normodense eosinophils. Eosinophils from 2 patients from this group expressed 20,000 125I-labeled monoclonal anti-CR1 antibody binding sites/cell. In another group of patients, 55 +/- 9% eosinophils spontaneously formed C3b-dependent rosettes that could not be enhanced by LTB4. In all patients, a mean of 16 +/- 9% eosinophils formed cation-dependent rosettes with C3bi-bearing intermediates that were inhibited by anti-CR3 antibody OKM1. All eosinophils stained with monoclonal antibodies against the alpha chain of CR3. There was no C3d-dependent rosette formation with eosinophils and no eosinophils stained with monoclonal anti-CR2 antibody. Thus, human eosinophils express CR1 and CR3. Since CR3 is required for the adhesion of granulocytes to surfaces and antibody-dependent cellular cytotoxicity of neutrophils, the interaction of C3 fragments with CR3 and CR1 on eosinophils may be of importance in eosinophil-mediated damage of opsonized targets. |
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