Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. |
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Authors: | Monica Calero Ruth N Collins |
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Institution: | Department of Molecular Medicine, Cornell University, Ithaca, New York 14853-6401, USA. |
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Abstract: | The regulation of membrane traffic involves the Rab family of Ras-related GTPases, of which there are a total of 11 members in the yeast Saccharomyces cerevisiae. Previous work has identified PRA1 as a dual prenylated Rab GTPase and VAMP2 interacting protein Martinic et al. (1999) J. Biol. Chem. 272, 26991-26998]. In this study we demonstrate that the yeast counterpart of PRA1 interacts with Rab proteins and with Yip1p, a membrane protein of unknown function that has been reported to interact specifically with the Rab proteins Ypt1p and Ypt31p. Yeast Pra1p/Yip3p is a factor capable of biochemical interaction with a panel of different Rab proteins and does not show in vitro specificity for any particular Rab. The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. |
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Keywords: | PRA1 YIP3 GDI Rab membrane traffic yeast YIP1 |
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