Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Streptomyces spp. |
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Authors: | M. S. El-Abyad M. A. El-Sayed A. R. El-Shanshoury Sabha M. El-Sabbagh |
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Affiliation: | (1) Department of Botany, Faculty of Science, Cairo University, Giza, Egypt;(2) Department of Botany, Faculty of Science, Tanta University, Egypt;(3) Department of Botany, Faculty of Science, Menoufeya University, Egypt |
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Abstract: | This investigation was designed to explore the potential of microbial antagonism in the control of some tomato diseases including bacterial, Fusarium and Verticillium wilts; early blight; bacterial canker. Three Streptomyces spp. were used: S. pulcher, S. canescens and S. citreofluorescens.The in vitro studies showed that an 80% concentration of the culture filtrate of either S. pulcher or S. canescens significantly inhibited spore germination, mycelial growth and spotulation of Fusarium oxysporum f.sp. lycopersici, Verticillium albo-atrum and Alternaria solani. The same concentration of filtrate of either S. pulcher or S. citreofluorescens was detrimental to the bacterial populations of Clavibacter michiganensis subsp. michiganensis and Pseudomonas solanacearum.The in vivo studies involved different treatments: soaking tomato seeds in filtrate of the antagonist prior to sowing, inoculation of the soil with the antagonist 7 days before sowing, and coating of tomato seeds with spores of the antagonist before sowing. The seed-coating treatment was the most effective in controlling all the pathogens at 42 and 63 days after sowing. Soil inoculation with the antagonist 7 days prior to sowing was less effective in controlling the tomato pathogens as compared to seed-coating. The seed-soaking treatment was the least effective in controlling the diseases concerned.The results also revealed that seed-coating with antagonistic Streptomyces spp. significantly improved tomato growth. |
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Keywords: | antagonism biocontrol growth response Lycopersicon esculentum phytopathogens seed-coating |
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