Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR |
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Authors: | Barbi Tommaso Drake Pascal M W Drever Matthew van Dolleweerd Craig J Porter Andrew R Ma Julian K-C |
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Institution: | (1) Molecular Immunology Unit, Centre for Infection, Division of Clinical Sciences, St. George’s University of London, Cranmer Terrace, room 2.148 Floor 2 Jenner Wing, London, SW17 0RE, UK;(2) Department of Molecular and Cell Biology, Foresterhill School of Medical Sciences, University of Aberdeen, Aberdeen, UK; |
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Abstract: | In this paper we describe the engineering and regeneration of transgenic tobacco plants expressing a recombinant plasma membrane-retained
antibody specific to microcystin-LR (MC-LR), the environmental toxin pollutant produced by cyanobacteria. The antibody was
created by a genetic fusion of the antigen binding regions of the microcystin-specific single chain antibody, 3A8, with the
constant regions from the murine IgG1κ, Guy’s 13, including a membrane retention sequence at the C-terminal end of the antibody
heavy chain. The antibody produced in the leaves was shown to be functional by binding to MC-LR in an ELISA with antibody
yields in transgenic plant leaves reaching a maximum of 1.2 μg g−1 leaf f.wt (0.005% total soluble protein). Antibody-MC-LR complexes formed in leaves after addition of MC-LR to hydroponic
medium around the roots of transgenic plant cultures. |
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