Salt adaptation in <Emphasis Type="Italic">Acinetobacter baylyi</Emphasis>: identification and characterization of a secondary glycine betaine transporter |
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Authors: | Miriam Sand Veronique de Berardinis Ana Mingote Helena Santos Stephan Göttig Volker Müller Beate Averhoff |
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Institution: | 1.Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences,Johann Wolfgang Goethe University Frankfurt,Frankfurt am Main,Germany;2.Genoscope,Institut de Génomique (CEA),Evry,France;3.Instituto de Tecnologia Química e Biológica,Universidade Nova de Lisboa,Oeiras,Portugal;4.Institute of Medical Microbiology and Infection Control,Goethe University,Frankfurt am Main,Germany |
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Abstract: | Members of the genus Acinetobacter are well known for their metabolic versatility that allows them to adapt to different ecological niches. Here, we have addressed
how the model strain Acinetobacter baylyi copes with different salinities and low water activities. A. baylyi tolerates up to 900 mM sodium salts and even higher concentrations of potassium chloride. Growth at high salinities was better
in complex than in mineral medium and addition of glycine betaine stimulated growth at high salinities in mineral medium.
Cells grown at high salinities took up glycine betaine from the medium. Uptake of glycine betaine was energy dependent and
dependent on a salinity gradient across the membrane. Inspection of the genome sequence revealed two potential candidates
for glycine betaine transport, both encoding potential secondary transporters, one of the major facilitator superfamily (MFS)
class (ACIAD2280) and one of the betaine/choline/carnitine transporter (BCCT) family (ACIAD3460). The latter is essential
for glycine betaine transport in A. baylyi. The broad distribution of ACIAD3460 homologues indicates the essential role of secondary transporters in the adaptation of
Acinetobacter species to osmotic stress. |
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