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Effect of peripheral nerve on the neurite growth from retinal explants in culture
Authors:Liu Li and So Kwokfai
Affiliation:Liu Li and So KwokfaiShanghai Institute of Cell Biology,Academia Sinica,Shanghai,ChinaDepartment of Anatomy,University of Hong Kong,Hong Kong
Abstract:The effect of peripheral nerve (PN) on neurite outgrowth from retinal explants of adult hamsters was examined. Cultures of retinal explants, and co-cultures of retinal explants and PN were performed using chick retinal basement membrane (BM) as substrate. The presence of PN increases the number and length of neurite outgrowth. In addition, a high proportion of neurites situated close to PN tend to grow towards it. Since there was no contact between retinal ex-plants and PN, we suggest that PN might secrete diffusible substances to attract the neurites to grow towards it.
Keywords:retinal explants  peripheral nerve  tissue culture  neurite outgrowth  adult hamster.
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