一株产纤维素酶真菌的筛选、鉴定及酶学性质初步研究

经过初筛和复筛从土样中分离出1株高产纤维素酶真菌SNB9，经形态学和ITS序列分析。鉴定为黑曲霉（Aspergu Uusniger）。生长条件的测定显示该菌生长范围偏酸。发酵后纤维素酶的最适作用pH在4．0—5．0，最适作用温度在45—55℃。滤纸酶活为9．29U／mL，C，酶活为23．69U／mL，CMCase酶活为38．23U／mL，β-葡萄糖苷酶活为65．52U／mL。发酵液中除了纤维素酶，还发现有辅助酶，包括木聚糖酶、淀粉酶、果胶酶、蛋白酶。

Screening and Characterization of Cellulase-Producing Fungus and Its Zymological Properties

A cellulase high-producing fungus strain SNB9 was isolated from soil samples after proto-screening and re-screening and characterized as Aspergillus niger after morphological and ITS sequencing analyses. Growth conditions tests indicated that it grew on meta-acidic range. And the most suitable reactive pH of the cellulase was 4.0～5.0, and temperature 45～55 ℃. The activities of FPA, C1, CMCase, and β-glycosidase were 9.29 U/mL, 23.69 U/mL, 38.23 U/mL, 65.52 U/mL respectively. Besides cellulase, it was found there were some other auxiliary enzymes in the fermented broth, including xylanase, amylase, pectinase, and protease.