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Selection of capsule deficient strains of Methylobacterium rhodesianum producing polyhydroxybutyrate |
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| Authors: | EJ Bormann M Roth W Linß M Leißner |
| Institution: | (1) Innovent e. V., Biomaterialien, Felsbachstr. 5, D-07745 Jena;(2) Hans-Knöll-Institut für Naturstoff-Forschung, Naturstoff-Technikum, Jena;(3) Institut für Anatomie, Friedrich-Schiller-Universität, Jena;(4) Fachhochschule Jena, Bereich Wirtschaftsingenieurwesen, German |
| Abstract: | Methylobacterium rhodesianum was cultivated using an extended fed-batch regime. After treatments with N-nitroso-methyl-urea or X-rays, colonies showing an irregular morphology or increased heavy-metal sensitivity were selected. Electron micrographs of cells of isolated strains demonstrated the loss of the glycocalyx. Selectants and the parental strain yielded identical PHB amounts. |
| Keywords: | capsule electron micrographs Methylobacterium polyhydroxybutyrate selection |
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