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PCR derived molecular markers and phylogenetic relationships in theSecale genus
Authors:J. C. Del Pozo  A. M. Figueiras  C. Benito  A. De La Peña
Affiliation:1. Departamento de Genética, Facultad de Biologia, Universidad Complutense Madrid, 28040, Madrid, Spain
Abstract:DNA from 22 different species, accessions, cultivars and lines included in theSecale genus were analyzed by the polymerase chain reaction (PCR), using as primers five pairs of oligonucleotides derived from specific sequences. A total of 42 amplified bands were considered, and some of them appeared to be potentially useful as molecular markers for some of the analyzed groups. These amplified bands were used to generate molecular phenograms inside theSecale genus.
Keywords:genetic markers  PCR band polymorphism  rye genetic diversity  rye phenograms
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