| 不同浓度激素对野生细叶结缕草愈伤组织的诱导与分化的影响 |
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| 引用本文： | 张瑜,杨知建,张志扬,张志飞. 不同浓度激素对野生细叶结缕草愈伤组织的诱导与分化的影响[J]. 生物技术通报, 2007, 0(2): 143-146 |
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| 作者姓名： | 张瑜 杨知建 张志扬 张志飞 |
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| 作者单位： | 1. 湖南农业大学农学院,长沙,410128
2. 湖南农业大学作物基因工程省重点实验室,长沙,410128 |
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| 基金项目： | 江苏省南京市科技局资助项目;留学回国科研人员启动费 |
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| 摘 要： | 以野生的细叶结缕草的匍匐茎为外植体,MS为基本培养基,研究2,4-D和BA及2,4-D和IAA不同浓度组合对其愈伤组织诱导与分化的影响。研究表明:(1)5.0mg/L的2,4-D对愈伤组织诱导有很大的促进作用;(2)匍匐茎诱导愈伤组织宜采用MS 2.0mg/L 2,4-D 4.0mg/L IAA,产生的愈伤组织分化情况也较好。
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| 关 键 词： | 野生 细叶结缕草 组织培养 愈伤组织 分化 |
| 修稿时间： | 2006-11-17 |
Effect of Hormone at Different Concentration on Inducement and Differentiation of Wild Z. tenuifolia Willd |
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| Zhang Yu,Yang Zhijian,Zhang Zhiyang,Zhang Zhifei. Effect of Hormone at Different Concentration on Inducement and Differentiation of Wild Z. tenuifolia Willd[J]. Biotechnology Bulletin, 2007, 0(2): 143-146 |
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| Authors: | Zhang Yu Yang Zhijian Zhang Zhiyang Zhang Zhifei |
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| Affiliation: | 1.College of Agronomy, Chongsha 410128 ;2.Crop gene Engineering Key Laboratory of Hunan Province HNA U, Chongsha 410128 |
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| Abstract: | The effect of 2,4-D(acetic acid)and 6-BA(6-benzyl aminopurine)at different concentration with MS medium on inducement and differentiation of wild and leaf-thin Z.tenuifolia Willd.'s stolon was studied.The results showed that:(1)2,4-D could improve the callu formation greatly at concentration of 5mg/L;(2)the proper culture medium for embryo callus inducement was MS 2.0 mg/L 2,4-D 4.0 mg/L IAA,in which the differentiation proceeded better. |
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| Keywords: | Wild Z.tenuifolia willd Tissue culture Callus tissue Differentiation |
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