Neuroprotection and Protein Damage Prevention by Estradiol Replacement in Rat Hippocampal Slices Exposed to Oxygen-Glucose Deprivation |
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Authors: | Helena?Cimarosti Ionara?R?Siqueira Lauren?L?Zamin Melissa?Nassif Rodrigo?Balk Rudimar?Frozza Carla?Dalmaz Carlos?Alexandre?Netto Email author&prev_q=Christianne?SalbegoEmail author" target="_blank">Christianne?SalbegoEmail author |
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Institution: | (1) Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, 90035-003 Porto Alegre, RS, Brazil;(2) Centro Universitário UNIVATES, Lajeado, RS, Brazil;(3) Departamento de Bioquímica, Instituto de Ciências Básicas da Sauda, UFRGS, RS, Brazil |
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Abstract: | Here we investigated the effects of estradiol replacement in ovariectomized female rats using hippocampal slices exposed to oxygen-glucose deprivation (OGD). OGD induced lactate dehydrogenase (LDH) release to the incubation medium, what was assumed as a parameter of cellular death. In the estradiol-treated group the LDH release was markedly decreased by 23% as compared to the vehicle-treated group. In attempt to study a possible mechanism by which estradiol acts, we investigated some parameters of oxidative stress. In both vehicle-treated and estradiol-treated groups, OGD significantly increased the free radical production by 34% and 16%, respectively, although no significant differences on total antioxidant capacity were observed. Interestingly, estradiol replacement prevented the significant reduction in tryptophan and tyrosine contents caused by OGD observed in vehicle-treated animals. Our results show that estradiol replacement in ovariectomized female rats decreases cellular susceptibility to an ischemic-like injury and suggest a role for the hormone on protein damage prevention. |
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Keywords: | Cerebral ischemia estradiol replacement hippocampal slices neuroprotection oxidative stress protein damage |
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