Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters |
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| Authors: | J Rouppe van der Voort E van der Vossen E Bakker H Overmars P van Zandvoort R Hutten R Klein Lankhorst J Bakker |
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| Institution: | (1) Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL;(2) Department of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands, NL;(3) Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands, NL |
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| Abstract: | Broad-spectrum resistance in potato to the potato cyst nematode (PCN) is commonly regarded as a complex inherited trait. Yet,
in this paper we show that, by use of a selected set of PCN test populations, broad-spectrum resistance to the species Globodera pallida can be fully ascribed to the action of two loci: Gpa5 and Gpa6. These loci were readily mapped by means of a strategy based on two steps. Firstly, the chromosomal localization of both
loci was assessed by use of an online catalogue of AFLP markers covering a substantial part of the potato genome (http://www.spg.wau.nl/pv/aflp/catalog.htm).
Subsequently the chromosomal regions of both loci were identified by means of CAPS markers based on RFLP insert sequences.
Locus Gpa5 explains at least 61% of the genetic variation. This locus maps to chromosome 5 on a region which has previously been shown to harbor resistance factors to viral (Nb, Rx2), fungal (R1) and nematodal (Gpa, Grp1) pathogens. The Gpa6 locus exhibits a minor effect on the resistance (24%) and acts additively to Gpa5. Interestingly, the Gpa6 locus maps to a region on chromosome 9 where, in the homoeologous tomato genome, the virus resistance gene Sw-5 resides as part of a resistance gene cluster. In potato, resistance to potato virus X has been reported in the vicinity of
this region. The map location of Gpa6 indicates the presence of a resistance gene cluster at the end of the long arm of chromosome 9 of potato.
Received: 10 January 2000 / Accepted: 31 January 2000 |
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| Keywords: | AFLP CAPS G rostochiensis Solanum tuberosum QTL mapping |
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