Glycogen synthesis by cell-free extracts from the dimorphic yeast Candida albicans |
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Authors: | Peter Orlean |
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Institution: | (1) Sub-department of Chemical Microbiology, Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW Cambridge, UK;(2) Present address: Institut für Botanik der Universität Regensburg, Universitätsstraße 31, 8400 Regensburg, Germany |
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Abstract: | A particulate glucosyltransferase prepared from budding and filamentous cultures of Candida albicans used uridine diphosphate glucose as sole glucosyl donor in a reaction (measured by following the incorporation of 14C]-glucose from UDP 14C]-glucose into polymer) stimulated by glucose-6-phosphate and inhibited by adenosine triphosphate and guanosine triphosphate. The radiolabelled reaction product was solubilized by -amylase, and, on oxidation with periodate followed by reduction with borohydride and acid hydrolysis, yielded erythritol and glycerol in the ratio of 4 to 1. The radiolabelled glucosyl residues were attached to an endogenous acceptor of high molecular weight. |
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