Effects of cultural conditions on the production of phenylalanine from a plasmid-harboring E. coli strain |
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Authors: | Cecilia Förberg Lena Häggström |
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Affiliation: | (1) Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-100 44 Stockholm, Sweden |
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Abstract: | Summary Phenylalanine production from E. coli KA 197/pJN6 (plasmid harboring genes for aro F, phe AFBR, AmpR and TcR) was studied under varying nutritional conditions in batch and continuous cultures. In batch culture experiments where growth was deliberately interrupted by limiting concentrations of sulphate and phosphate the phenylalanine production continued from the non-growing cells. However, the depletion of phosphate resulted in an immediate cessation of phenylalanine production but thereafter a low specific rate of phenylalanine formation resumed, while the decrease in specific rate of product formation was less after sulphate depletion. In the chemostat experiments, however, phosphate limitation was the only case where the specific rate of phenylalanine formation remained constant, while at the corresponding time in sulphate and glucose limited chemostats it was declining respectively had ceased. |
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