Molecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars |
| |
Authors: | F Chen Z H He X C Xia L Q Xia X Y Zhang M Lillemo C F Morris |
| |
Institution: | Chinese Academy of Agricultural Sciences (CAAS), Institute of Crop Science/National Wheat Improvement Center, Zhongguancun South Street 12, Beijing 100081, China. |
| |
Abstract: | Kernel hardness that is conditioned by puroindoline genes has a profound effect on milling, baking and end-use quality of
bread wheat. In this study, 219 landraces and 166 historical cultivars from China and 12 introduced wheats were investigated
for their kernel hardness and puroindoline alleles, using molecular and biochemical markers. The results indicated that frequencies
of soft, mixed and hard genotypes were 42.7, 24.3, and 33.0%, respectively, in Chinese landraces and 45.2, 13.9, and 40.9%
in historical cultivars. The frequencies of PINA null, Pinb-D1b and Pinb-D1p genotypes were 43.8, 12.3, and 39.7%, respectively, in hard wheat of landraces, while 48.5, 36.8, and 14.7%, respectively,
in historical hard wheats. A new Pinb-D1 allele, designated Pinb-D1t, was identified in two landraces, Guangtouxianmai and Hongmai from the Guizhou province, with the characterization of a glycine
to arginine substitution at position 47 in the coding region of Pinb gene. Surprisingly, a new Pina-D1 allele, designated Pina-D1m, was detected in the landrace Hongheshang, from the Jiangsu province, with the characterization of a proline to serine substitution
at position 35 in the coding region of Pina gene; it was the first novel mutation found in bread wheat, resulting in a hard endosperm with PINA expression. Among the
PINA null genotypes, an allele designed as Pina-D1l, was detected in five landraces with a cytosine deletion at position 265 in Pina locus; while another novel Pina-D1 allele, designed as Pina-D1n, was identified in six landraces, with the characterization of an amino acid change from tryptophan-43 to a ‘stop’ codon
in the coding region of Pina gene. The study of puroindoline polymorphism in Chinese wheat germplasm could provide useful information for the further
understanding of the molecular basis of kernel hardness in bread wheat. |
| |
Keywords: | Bread wheat (Triticum aestivum L ) Kernel hardness Puroindolines Pina and Pinb alleles |
本文献已被 PubMed SpringerLink 等数据库收录! |
|