Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers |
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| Authors: | Ainong Shi Pengyin Chen Dexiao Li Cuiming Zheng Bo Zhang Anfu Hou |
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| Institution: | (1) Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA;(2) Present address: Institute of Genomic Diversity, Cornell University, Ithaca, NY 14853, USA;(3) Present address: College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, People’s Republic of China;(4) Present address: Syngenta Seeds Inc, 3 Ultraway Dr, Highland, IL 62249, USA;(5) Present address: Agriculture and Agri-Food Canada, Unit 100-101 Route 100, Morden, MB, Canada, R6M 1Y5; |
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| Abstract: | Seven strains of Soybean mosaic virus (SMV) and three independent resistance loci (Rsv1, Rsv3, and Rsv4) have been identified in soybean. The objective of this research was to pyramid Rsv1, Rsv3, and Rsv4 for SMV resistance using molecular markers. J05 carrying Rsv1 and Rsv3 and V94-5152 carrying Rsv4 were used as the donor parents for gene pyramiding. A series of F2:3, F3:4, and F4:5 lines derived from J05 × V94-5152 were developed for selecting individuals carrying all three genes. Eight PCR-based markers
linked to the three SMV resistance genes were used for marker-assisted selection. Two SSR markers (Sat_154 and Satt510) and
one gene-specific marker (Rsv1-f/r) were used for selecting plants containing Rsv1; Satt560 and Satt063 for Rsv3; and Satt266, AI856415, and AI856415-g for Rsv4. Five F4:5 lines were homozygous for all eight marker alleles and presumably carry all three SMV resistance genes that would potentially
provide multiple and durable resistance to SMV. |
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| Keywords: | Gene pyramiding Marker-assisted selection Resistance gene Soybean Soybean mosaic virus |
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