中国6株狂犬病病毒街毒株全基因组测序与分析

实验研究中对分离于中国的6株狂犬病病毒街毒株进行了全基因组测序,对基因组的5个结构基因(N、P、M、G和L)的核苷酸和推断的氨基酸序列以及非编码区序列进行了分析与比较,并与来自GenBank的40株毒株从全基因组水平进行了分子进化分析。所测6株中国狂犬病病毒街毒株的全基因组核苷酸序列长度介于11 907 nt(CQ92)和11 924 nt(SH06和gg4)之间,基因组结构相同,用全基因组和不同的结构基因构建的进化树拓扑结构相似,基因组3′和5′末端高度保守而且末端11个核苷酸互补配对,5个结构基因的保守性依次是NLMGP,核苷酸同源性的最小值依次分别是81.9%、81.7%、80.7%、78.3%和76.7%。

Sequencing and analysis of complete genome of six street Rabies virus isolates from China

The complete genome sequences of 6 new street virus isolates from China were determined,and the complete nucleotide and deduced amino acid sequences of five structural genes（N,P,M,G and L） and non-structural genes were analyzed then the obtainable data were compared with those of 40 complete genome sequences from GenBank so as to analyze the evolution level.The results showed that all of 6 genomes of rabies virus isolates from China have the same structural organization although their lengths varied between 11 907 nt（CQ92） and 11 924 nt（SH06 and gg4）.The toplogies of the reconstructed tree in phylogenetic analyses are similar,the high conservative area is located at the 3′and 5′ends of the genome,and 11 terminal nucleotides of 3′ and 5′ end match each other.The percentage expresses the extent of homology of gene,the homology of gene is in the order of NLMGP（81.9%,81.7%,80.7%,78.3% and 76.7%,respectively）.