Identification and characterization of maize pathogenesis-related proteins. Four maize PR proteins are chitinases |
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Authors: | William Nasser Marc de Tapia Serge Kauffmann Shideh Montasser-Kouhsari Gérard Burkard |
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Institution: | (1) Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67000 Strasbourg, France |
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Abstract: | Eight pathogenesis-related proteins extractable at pH 2.8 were found to accumulate in maize leaves after mercuric chloride treatment or brome mosaic virus infection. These proteins were called PRm (pathogenesis-related maize) proteins. Seven PRm proteins were purified to homogeneity by preparative polyacrylamide gel electrophoresis and their amino acid compositions determined. Estimated molecular weights in SDS-containing gels were: PRm 1 14.2 kDa; Prm 2 16.5 kDa; PRm 3 and PRm 4 25 kDa; PRm 6b 30.5 kDa; PRm 6a 32 kDa; PRm 7 34.5 kDa. Antisera raised against either PRm 3 or PRm 4 reacted specifically each with PRm 3 or PRm 4. Antisera raised against PRm 6b reacted with PRm 6b as well as with PRm 6a and antisera against PRm 7 reacted with PRm 7 and PRm 5. Tobacco anti-PR 1b antisera reacted with maize PRm 2.Chitinase (poly1,4-(N-acetyl- -D-glucosamide)]glycanhydrolase, EC 3.2.1.14) activity was found for PRm 3, PRm 4, PRm 5, and PRm 7. |
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Keywords: | Zea mays pathogenesis mercuric chloride brome mosaic virus antimicrobial enzymes |
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