Bovine trophoblastic cell vesicle attachment to polarized endometrial epithelial cells in vitro |
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Authors: | Linda Munson Joanna E Ellington Donald H Schlafer |
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Institution: | (1) Department of Pathology, New York State College of Veterinary Medicine, 14853 Ithaca, New York;(2) Department of Animal Science, Cornell University, 14853 Ithaca, New York;(3) Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, P.O. Box 1071, 37901 Knoxville, TN |
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Abstract: | Summary Interactions between bovine trophoblastic cell vesicles and bovine endometrial epithelial cells were investigated by light
and electron microscopy and lectin histochemistry in a cell culture model of early blastocyst attachment. Primary lines of
bovine endometrial epithelial cells were polarized by subculturing on substrata and maintaining cultures at the air-medium
interface. Trophoblastic cell vesicles were obtained from elongated Day 14 blastocysts. In co-cultures, trophoblastic cell
vesicles adhered to endometrial epithelial cells through microvillus interdigitation and formation of primitive membrane junctional
complexes. After 3 d in co-culture, a multilayered cellular plaque formed at the trophoblastic cell-endometrial epithelial
cell interface. The type of cells contributing to this local proliferative response could not be identified specifically as
trophoblastic or endometrial cells, and areas of membrane fusion between cells were noted. Ultrastructural features of vesicle
adhesion in cultures were similar to features of conceptus attachment in vivo. Lectins bound to apical membranes of trophoblastic
cells and endometrial epithelial cells in all locations except contact sites between vesicles and endometrial cells. These
findings suggest that local cellular proliferation and membrane fusion between trophoblastic and endometrial epithelial cells
may be early events in conceptus implantation in the cow and these events can be reproduced in culture.
This work was supported by a grant from U.S. Department of Agriculture Animal Health and Disease Program, Washington, DC. |
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Keywords: | trophoblast endometrium implantation bovine cell culture |
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