Impact of hypoxia on the growth and alkaloid accumulation in <Emphasis Type="Italic">Catharanthus roseus</Emphasis> cell suspension |
| |
Authors: | Mohammed Mourad Senoussi Bassa Nora Crèche Joêl |
| |
Institution: | (1) University of Larbi Ben M’hidi, Oum El Bouaghi, Algeria;(2) Department of Plant Molecular and Cell Biology, Faculty of Pharmacy, 31 avenue monge, 37200 Tours, France |
| |
Abstract: | The Madagascar periwinkle (Catharanthus roseus) produces numerous indole alkaloids, several of which have an important pharmaceutical uses such as ajmalicine, vinblastine
and vincristine. The relationship between hypoxia and ajmalicine production in a cell suspension culture of C. roseus were investigated during the cycle of cell culture, in correlation with the effects on growth. The results show that the
lack of oxygenation in C20D cells provokes a very strong inhibition in accumulation of the alkaloids and of other possible
substances. Moreover, the addition of loganin, a metabolic intermediate of the biosynthetic pathway, in the culture medium
of cells subjected to hypoxia restored the alkaloid production. Also, the results showed that the addition of benzyladenine
(BA) to the culture medium increased the ajmalicine production and that the inhibitory effect of hypoxia was almost absent
in these conditions. Therefore, it could be suggested that BA can without doubt decrease the effects of the hypoxia and increase
the ajmalicine production in periwinkle cell suspensions. |
| |
Keywords: | Catharanthus roseus Ajmalicine Hypoxia Cytokinin Loganin |
本文献已被 SpringerLink 等数据库收录! |
|