Electrospray ionization mass spectrometry analysis of polyisoprenoid alcohols via Li+ cationization |
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Authors: | D'Alexandri Fabio Luiz Gozzo Fabio Cesar Eberlin Marcos Nogueira Katzin Alejandro Miguel |
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Institution: | Department of Parasitology, Institute of Biomedical Sciences, University of S?o Paulo, 05508-900 S?o Paulo, SP, Brazil. |
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Abstract: | Direct analysis of polyisoprenoids by electrospray ionization mass spectrometry (ESI-MS) often produces poor results requiring off-line time and sample-consuming derivatization techniques. We describe a simple ESI-MS approach for the direct analysis of polyisoprenoids using several dolichols and polyprenols with different chain sizes as proof-of-principle cases. Lithium iodide is used to promote cationization by intense formation of M+Li]+ adducts. Thus, detection of polyisoprenoids with mass determination can be performed with high sensitivity (limit of detection LOD] approximately 100 rhoM), whereas characteristic collision-induced dissociations observed for both dolichols and polyprenols permit investigation of their structure. Using ESI(Li+)-MS and ESI(Li+)-MS/MS analysis, we screened for polyprenol products of an octaprenyl pyrophosphate synthase of Plasmodium falciparum and dolichols in a complex mixture of compounds produced by Leishmania amazonensis and P. falciparum. |
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Keywords: | Mass spectrometry Polyisoprenoids Polyprenols Dolichols Plasmodium falciparum Leishmania amazonensis Lithium |
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