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Expression of a green fluorescence protein-carrier protein into mouse spermatozoa |
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| Authors: | Mogas Teresa Fernández-Novell Josep M Palomo Maria Jesús Otaegui Pedro J Gomis Roger R Ballester Joan Izquierdo Dolors Guinovart Joan J Ferrer Joan C Rigau Teresa Rodríguez-Gil Joan E |
| Affiliation: | Unitat de Reproducció, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain. |
| Abstract: | Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7days to, at least, 16days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins. |
| Keywords: | Green fluorescence protein Mature spermatozoa Protein tracking |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |