Multicomponent Investigations of the Hydrogen Peroxide- and Hydroxyl Radical-Scavenging Antioxidant Capacities of Biofluids: The Roles of Endogenous Pyruvate and Lactate Relevance to Inflammatory Joint Diseases |
| |
Authors: | Herman Herz David R Blake Martin Grootveld |
| |
Institution: |
a The Inflammation Research Group, Bone and Joint Research Unit, The London Hospital Medical College, London, UK |
| |
Abstract: | High field proton (1H) nuclear magnetic resonance (NMR) analysis of biofluids (healthy human blood sera and inflammatory knee-joint synovial fluids) has been employed to evaluate the hydrogen peroxide (H2O2)- and hydroxyl radical (°OH)- scavenging antioxidant capacities of a range of polar, low-molecular-mass endogenous metabolites therein. Data obtained indicate that consumption of H2O2 by pyruvate (generating acetate and CO2 via an oxidative decarboxylation reaction) and °OH radical by lactate (generating pyruvate, and subs quently acetate and CO2) may serve to protect alternative biofluid components (e.g., macromolecules) against reactive oxygen species-mediated oxidative damage in vivo. The mechanistic, physiological and potential therapeutic implications of these results are discussed with special reference to inflammatory joint diseases. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|