Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris |
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| Authors: | Wenhui Zhang Mehmet Inan Michael M. Meagher |
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| Affiliation: | (1) Biological Process Development Eacility, Department of Chemical Engineering, University of Nebraska-Lincoln, 68583-0919 Lincoln, Nebraska, USA |
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| Abstract: | Fermentation strategies for recombinant protein production inPichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail. |
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| Keywords: | Pichia pastoris methylotrophic yeast fed-batch growth fermentation optimization growth modeling recombinant protein expression |
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