Influence of monosaccharides on the fibrillogenesis of type I collagen |
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| Authors: | A N Rathi S Padmavathi G Chandrakasan |
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| Institution: | Biological Sciences Laboratory, Central Leather Research Institute, Madras, India. |
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| Abstract: | The in vitro self-assembly of Type I rat tail tendon collagen in the presence of monosaccharides was studied turbidimetrically. D(+)glucose and D(+)mannose delayed fibril formation significantly while D(-)fructose showed no variation. The effect of glucose was concentration dependent. Binding of glucose during fibrillogenesis was shown by the incorporation of 14C]glucose and also by SDS-polyacrylamide slab gel electrophoresis of the product. The results indicate that in vitro nonenzymatic glycosylation can take place in the short duration required for fibril formation. |
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