Direct and indirect regeneration of plants from internodal and leaf expiants of<Emphasis Type="Italic">Hypencum bupleuroides</Emphasis> gris |
| |
Authors: | Cüneyt çirak Ali Kemal Ayan Kudret Kevsero?lu |
| |
Institution: | 1.Faculty of Agriculture, Department of Agronomy,University of Ondokuz Mayis,Samsun/Turkey;2.the High School of Profession of Bafra,University of Ondokuz Mayis,Samsun/Turkey |
| |
Abstract: | Species of the genusHypencum are of considerable interest worldwide because of their medicinal properties.In- vitro culture is a useful tool for both multiplication of the genus and studying its economically important secondary metabolites.
Here, we present an effectivein- vitro propagation method forH. bupleuroides. Leaf and internodal expiants excised from 9-week-old,in vitro-germinated seedlings were cultured on a Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 1.0 or 0.1 mg
L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D; 1.0 or 0.1 mg L1). Depending on the BA and 2,4-D combination used, these cultures produced adventitious shoot buds directly on the surfaces
of both types of explants as well as excessive calli. Numerous shoots were obtained when the calli from both expiant types
were cultured on an MS medium supplemented with 2 mg L-1 BA. Internodal expiants were more responsive than leaf tissues to direct and indirect plant regeneration. After shoots that
regenerated from either the calli or the expiant surface were excised, rooting was best on an MS medium lacking any growth
hormones. These rooted plants were then acclimatized under greenhouse conditions, and 90% of regenerants had survived. Ours
is the first report ofin- vitro plant regeneration fromH. bupleuroides. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|