| 人体高必需氨基酸编码蛋白转基因马铃薯的获得及RT-PCR分析 |
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| 引用本文： | 宋东光,于湄,王惠珍,汪训明. 人体高必需氨基酸编码蛋白转基因马铃薯的获得及RT-PCR分析[J]. 生物学杂志, 2002, 18(6): 16-19 |
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| 作者姓名： | 宋东光 于湄 王惠珍 汪训明 |
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| 作者单位： | 1. 佛山科学技术学院农学系,广东,佛山,528231
2. 复旦大学遗传所,上海,200433 |
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| 基金项目： | 国家 863专项基金，佛山科学技术学院博士启动基金，广东省自然科学基金 (0 0 0 0 1 4 )的资助 |
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| 摘 要： | 人口的增长增加了对粮食的需求而提高食物的蛋白质营养将能够缓解这一状况。将人体高必需氨基酸蛋白基因（HEAAE）导入马铃薯以改善马铃薯主食地区的蛋白质营养的研究得到了重视。经过PCR和RT-PCR分析，HEAAE与GUS融合基因在马铃薯块茎专一性高表达class Ipatatin基因启动子驱动下在转基因马铃薯中获得了稳定表达。
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| 关 键 词： | 人体高必需氨基酸编码蛋白 RT－PCR 转基因马铃薯 |
| 文章编号： | 1008-9632(2002)06-0016-04 |
| 修稿时间： | 2002-06-27 |
RT-PCR analysis of transgenic potatoes by human high essential amino acids encoding gene |
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| SONG Dong-guang,YU Mei,WANG Hui-zhen,WANG Xun-ming. RT-PCR analysis of transgenic potatoes by human high essential amino acids encoding gene[J]. Journal of Biology, 2002, 18(6): 16-19 |
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| Authors: | SONG Dong-guang YU Mei WANG Hui-zhen WANG Xun-ming |
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| Affiliation: | SONG Dong-guang1,YU Mei1,WANG Hui-zhen1,WANG Xun-ming2 |
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| Abstract: | Rapid growth of global population demands more food and this might be alleviated by improving protein nutrition levels in field crops.One important approach is to introduce a foreign gene encoding high essential amino acids(hEEAs) into potatoes for enhancing protein nutrition level in areas consuming potatoes as the main staple.The stable expression of GUS-HEAAE fusion gene directed by promoter of a tuber-specific clss I patatin gene was observed in transgenic potato by RT-PCR analysis. |
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| Keywords: | RT-PCR HEAAE transgenic potato |
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