Hydrogen peroxide increases the phagocytic function of human neutrophils by calcium mobilisation |
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Authors: | I. Bejarano M. P. Terrón S. D. Paredes C. Barriga A. B. Rodríguez J. A. Pariente |
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Affiliation: | (1) Department of Physiology, Faculty of Science, University of Extremadura, Av. De Elvas s/n, Badajoz, 06071, Spain |
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Abstract: | We have studied the effect of exogenous administration of hydrogen peroxide (H2O2) on phagocytic activity of human neutrophils. The treatment of cells with increasing concentrations of H2O2 evoke a significant elevation of phagocytic function assayed as phagocytic index, percentage and efficiency; and was similar to that induced by the calcium mobilising agonist formyl-methionyl-leucyl-phenylalanine (fMLP). This stimulatory effect was reduced by pre-treatment of neutrophils with catalase and abolished in neutrophils loaded with the intracellular calcium quelator dimethyl BAPTA. In the absence of extracellular calcium, treatment of cells with H2O2 resulted in a increase in [Ca2+] i , indicating the release of calcium from intracellular stores. H2O2 abolished the typical calcium release stimulated by the physiological agonist fMLP, while depletion of agonist-sensitive calcium pools by fMLP was able to prevent H2O2-induced calcium release. We conclude that H2O2 induces calcium release from agonist-sensitive stores and consequently increase the phagocytosis process. |
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Keywords: | Hydrogen peroxide phagocytic function neutrophils calcium |
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