Physiological and proteomic analysis of young rice leaves grown under nitrogen-starvation conditions |
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Authors: | Sang Gon Kim Yiming Wang Jingni Wu Kyu Young Kang Sun Tae Kim |
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Institution: | (1) Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Korea;(2) Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, 660-701, Korea;(3) Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea; |
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Abstract: | Rice grown in anaerobic waterlogged soil accumulates ammonium as a major source of nitrogen (N). We have compared the physiological
symptoms of rice seedlings subjected to N-starvation stress with those receiving sufficient N, based on measurements of shoot/root
length and weight and an analysis of protein expression patterns. N starvation marginally increased root growth but notably
decreased shoot biomass. N uptake was reduced by >50% in the roots and shoots of N-starved seedlings. To better understand
the mechanism of N starvation in rice, we performed a comparative proteome analysis of proteins isolated from rice leaves.
Twenty-five differentially expressed proteins were analyzed by matrix-assisted laser desorption/ionization time-of-flight
(TOF) mass spectrometry and electron spray ionization quadrupole TOF. Functional analysis of the N-starvation response proteins
suggested their involvement in protein synthesis and fate, metabolism, and defense. These results indicate that these proteins
may play important roles in regulating the plant’s complex adaptation responses for N use during N starvation. The proteins
may be useful for further characterization of protein function in plant N nutrition. |
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