Mutagenesis with methyl methanesulfonate in mouse |
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| Authors: | J Moutschen |
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| Affiliation: | 1. Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seoul, Korea;2. Department of Thoracic and Cardiovascular Surgery, Seoul Metropolitan Government–Seoul National University (SMG-SNU) Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea;3. Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seoul, Korea;1. Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA;2. Department of Physiology and Functional Genomics, University of Florida, Gainesville, FL 32610, USA;1. Dipartimento di Scienze delle Produzioni Vegetali Sostenibili, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza 29122, Italy;2. Dipartimento di Biologia, Università di Bari “Aldo Moro”, via E. Orabona 4, Bari 70125, Italy;1. College of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China;2. Crop Institute, Anhui Academy of Agricultural Sciences/Anhui Key Laboratory of Crops Quality Improving, Hefei 230031, P.R.China;1. Department of Physical Chemistry and Applied Thermodynamics, EPS, Edificio Leonardo da Vinci, Campus de Rabanales, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14071 Córdoba, Spain;2. Department of Sciences and Agricultural and Forest Resources, Edificio C-4 “Celestino Mutis”, Campus de Rabanales, Universidad de Córdoba, Crta. Madrid-Cádiz Km 396, 14071 Córdoba, Spain;3. Crop Protection Area, IFAPA, Centro Alameda del Obispo, CAP Junta de Andalucía, Avda. Menéndez Pidal s/n, Apdo. 3092, 14080 Córdoba, Spain |
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| Abstract: | The toxicity of methyl methanesulfonate was tested in several mouse strains as a preliminary to the design of further experiments on mutagenesis. Doses of 30 and 60 mg/kg were selected for further investigations.By direct cytological observations, it was found that the end of pachytene shows a special sensitivity. The doses induced dominant lethal mutations. Treatment of post-meiotic stages resulted in a considerable amount of deciduomata. In contrast the proportion of deciduomata arising from meiotic or pre-meiotic stages was low.Experiments involving the sex-linked marker Tabby and XO females were designed to test chromosome non-disjunction. The frequency of non-disjunctions induced in our strain is discussed.From these experiments it appears that methyl methanesulfonate is efficient for inducing structural changes in chromosomes of the mouse. This confirms several reports in the literature. |
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